graphpad prism 5 four parameter non linear regression curve

1 The relative potency is generally set to one for the reference curve (known agent) and the scaling factor used to transform the reference curve into the test curve (unknown agent) is the relative potency of the unknown agent. Two curves are defined to be parallel when one function is obtained from the other by a scaling factor either to the right or to the left on the x-axis, ƒ(x) = ƒ(rx), where x is the dose and r is the scaling factor, or relative potency. Parallel line analysis of dose response data sets with a constrained global 4-parameter curve fit. Testing for parallelism is a prerequisite to calculate the relative potency of a compound and plays an important role in many pharmaceutical drug development applications such as drug comparison, analyte confirmation, crossreactivity, interfering substances, matrix compensation, concentration estimation, and inhibitory studies.įigure 1.

Parallelism methods allow the user to establish if the biological response to two substances is similar or if two biological environments give similar dose-response curves to the same substances. PLA is commonly used to compare dose-response curves where there is no direct measurement of a product, but rather an effect is measured (Figure 1). In laboratories operating under GMP (good manufacturing practice) and GLP (good laboratory practice) regulations, biological assays are frequently analyzed with the help of parallel line analysis (PLA). High-Content Screening with AgileOptix Technology.PROTEIN DETECTION, QUANTITATION, ANALYSIS.NUCLEIC ACID (DNA/RNA) DETECTION & ANALYSIS.

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Graphpad prism 5 four parameter non linear regression curve